Recombinant Human UBE2I/Ubc9 His (N-Term) Protein

Name: Recombinant Human UBE2I/Ubc9 His (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-35094

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35094

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His (N-Term)

Conjugate

Unconjugated

Applications

SDS-PAGE

View all UBE2I/Ubc9 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 158 amino acids corresponding to UBE2I/Ubc9 and 8 aa vector sequence including 6x His tag at N-terminus Source: E. coli

Amino Acid Sequence: MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of UBE2I/Ubc9 as determined by LAL method.

Predicted Molecular Mass

19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UBE2I/Ubc9 His (N-Term) Protein

SDS-PAGE: Recombinant Human UBE2I/Ubc9 His (N-Term) Protein [NBP2-35094]

SDS-Page: Human UBE2I/Ubc9 His (N-Term) Protein [NBP2-35094]

Formulation, Preparation and Storage

NBP2-35094

Formulation	A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution	Please contact technical support for detailed reconstitution instructions.
Format	Carrier-Free
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2I/Ubc9

Human Ubiquitin Conjugating Enzyme E2 I (UBE2I)/SUMO-conjugating enzyme UBC9 (UBC9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. UBC9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I kappa B alpha, and PML without the requirement of an E3 ligase.

Long Name

Ubiquitin-conjugating Enzyme E2I

Alternate Names

p18, SUMO-protein Ligase, Ubc9, UBCE9

Gene Symbol

UBE2I

Additional UBE2I/Ubc9 Products

Product Documents for Recombinant Human UBE2I/Ubc9 His (N-Term) Protein

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COA

Product Specific Notices for Recombinant Human UBE2I/Ubc9 His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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