Recombinant Human VRK3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98944

Novus Biologicals, part of Bio-Techne
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NBP1-98944-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all VRK3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-474 of Human VRK3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMISFCPD CGKSIQAAFK FCPYCGNSLP VEEHVGSQTF VNPHVSSFQG SKRGLNSSFE TSPKKVKWSS TVTSPRLSLF SDGDSSESED TLSSSERSKG SGSRPPTPKS SPQKTRKSPQ VTRGSPQKTS CSPQKTRQSP QTLKRSRVTT SLEALPTGTV LTDKSGRQWK LKSFQTRDNQ GILYEAAPTS TLTCDSGPQK QKFSLKLDAK DGRLFNEQNF FQRAAKPLQV NKWKKLYSTP LLAIPTCMGF GVHQDKYRFL VLPSLGRSLQ SALDVSPKHV LSERSVLQVA CRLLDALEFL HENEYVHGNV TAENIFVDPE DQSQVTLAGY GFAFRYCPSG KHVAYVEGSR SPHEGDLEFI SMDLHKGCGP SRRSDLQSLG YCMLKWLYGF LPWTNCLPNT EDIMKQKQKF VDKPGPFVGP CGHWIRPSET LQKYLKVVMA LTYEEKPPYA MLRNNLEALL QDLRVSPYDP IGLPMVP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human VRK3 His Protein

SDS-PAGE: Recombinant Human VRK3 His Protein [NBP1-98944]

SDS-PAGE: Recombinant Human VRK3 His Protein [NBP1-98944]

SDS-Page: Recombinant Human VRK3 Protein [NBP1-98944] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98944
Formulation 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VRK3

VRK3, also known as inactive serine/threonine-protein kinase, belongs to the protein kinase superfamily. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. VRK3 is widely expressed in human tissues and its protein localizes to the nucleus. Recombinant human VRK3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

inactive serine/threonine-protein kinase VRK3, serine/threonine-protein kinase VRK3, Serine/threonine-protein pseudokinase VRK3, vaccinia related kinase 3, Vaccinia-related kinase 3

Gene Symbol

VRK3

Additional VRK3 Products

Product Documents for Recombinant Human VRK3 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VRK3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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