Recombinant Human XCL1/Lymphotactin Protein

Name: Recombinant Human XCL1/Lymphotactin Protein
Brand: Novus Biologicals
SKU: NBP2-34948

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34948

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Product Datasheet / COA

Catalog #

Availability

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P47992

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all XCL1/Lymphotactin Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 92 amino acids corresponding to XCL1/Lymphotactin Source: E. coli

Uniprot ID: P47992

Amino Acid Sequence: GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of XCL1/Lymphotactin as determined by LAL method.

Predicted Molecular Mass

10.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Lymphotactin Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-34948

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: XCL1/Lymphotactin

Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1. Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60 % amino acid sequence homology with the murine and rat protein.

Long Name

Chemokine [C Motif] Ligand 1

Alternate Names

ATAC, Lymphotactin, SCM-1 alpha, Lymphotactin/XCL1, XCL1

Gene Symbol

XCL1

UniProt