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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse IgG IgA and IgM whole molecule

Specificity

This antibody was pre-adsorbed against Bovine, Horse, and Human Serum Proteins. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1% of the target signal.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)

Application
Recommended Usage

ELISA

1:200000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA, SDS-PAGE, and Dot Blot, and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgA/IgG/IgM (H+L)

Additional IgA/IgG/IgM (H+L) Products

Product Documents for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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