Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73479
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Mouse IgG IgA and IgM whole molecule
Specificity
This reagent is suitable for the detection of all mouse isotypes and chain combinations.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM
Scientific Data Images for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC]
Dot Blot: Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC] [NBP1-73479] - Dot Blot for Goat anti-Mouse IgA/ IgG/IgM (H+L) Secondary antibody [FITC].Lane 1A: 100 ng Ms IgG.Lane 1B: 100 ng Ms IgA.Lane 1C: 100 ng Ms IgM .Lanes 2-5: serial dilution 3 fold of respective Antigen.Primary Antibody: none. Secondary Antibody: Goat Anti-Goat anti-Mouse IgA/ IgG/IgM (H+L) Secondary antibody [FITC] at 1:1,000 dilution at RT for 1 hour. Block at RT for 30 minutes.
Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC]
Dot Blot for Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated. Lane 1A: 100 ng Ms IgG. Lane 1B: 100 ng Ms IgA. Lane 1C: 100 ng Ms IgM. Lanes 2-5: serial dilution 3 fold of respective Antigen. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC] at 1:1,000 dilution at RT for 1 hour. Block at RT for 30 minutes.Applications for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC]
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgA/IgG/IgM (H+L)
Additional IgA/IgG/IgM (H+L) Products
Product Documents for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC]
Product Specific Notices for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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