Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB7459
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human IgE
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with human IgE. Cross-reactivity with other human immunoglobulins and light chains is less than 1%.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:100000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:2000 - 1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Phosphate Buffered Saline (PBS) containing 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgE Heavy Chain
Alternate Names
Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon
Additional IgE Heavy Chain Products
Product Documents for Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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