Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45061
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # EM-07
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Fusion protein of human IgG Fc fragment.
Specificity
This secondary antibody reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Store in the dark. Avoid prolonged exposure to light.
Scientific Data Images for Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [FITC]
Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [FITC] [NBP1-45061] - IgG kappa light chain (1), IgG alpha light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with antibody against IgG kappa light chain (A) and antibody against IgG Fc fragment (B).
Applications for Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [FITC]
Application
Recommended Usage
ELISA
1:2000
Flow Cytometry
1-4 ug/ml
Western Blot
1:1000
Application Notes
This antibody is useful in Western Blot, Immunocytochemistry, ELISA.
Formulation, Preparation, and Storage
Purification
Size Exclusion Chromatography
Formulation
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
0.9% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [FITC]
Product Specific Notices for Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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