Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51846
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human IgG Fc antiserum was solid phase adsorbed to ensure class specificity.
Reactivity Notes
Minimum reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep.
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications for Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:1000 - 1:20000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:1000 - 1:20000
Application Notes
Suggested starting dilutions are: ELISA: Coating: 1:100 - 1:500, Primary: 1:1000 - 1:30,000. Western Blots: Colorimetric: 1:1,000 - 1:10,000, Chemiluminescent: 1:1,000 - 1:30,000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Phosphate Buffered Saline (PBS)
Format
Pre-adsorbed
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)
Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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