Key Product Details

Species Reactivity

Sheep

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Rabbit anti-Sheep IgG Fc fragment Secondary Antibody was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.

Specificity

No reaction was observed against Sheep IgG F(ab).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Sheep IgG Fc Secondary Antibody

ELISA: Rabbit anti-Sheep IgG Fc Secondary Antibody [NBP1-72768]

ELISA: Rabbit anti-Sheep IgG Fc Secondary Antibody [NBP1-72768]

ELISA: Rabbit anti-Sheep IgG Fc Secondary Antibody [NBP1-72768] - ELISA Results of Rabbit anti-Sheep IgG Fc Secondary antibody tested against purified Sheep IgG Fc. Each well was coated in duplicate with 1.0 ug of Sheep IgG Fc [Red Line] and Sheep IgG, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.

Applications for Rabbit anti-Sheep IgG Fc Secondary Antibody

Application
Recommended Usage

ELISA

1:10000 - 1:200000

Immunohistochemistry

1:500 - 1:2500

Western Blot

1:1000 - 1:5000
Application Notes
This product has been tested by ELISA and dot blot and is assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Sheep IgG Fc Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Sheep IgG Fc Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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