Key Product Details

Species Reactivity

Canine

Applications

Dot Blot, ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Dog IgG F(c) fragment

Specificity

No reaction was observed against Dog IgG F(ab).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG, Dog IgG F(c) and Dog Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Canine IgG Fc Secondary Antibody

Dot Blot: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - Antigen: Dog IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Dog IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.
ELISA: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773]

ELISA: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773]

ELISA: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - ELISA results of purified Rabbit anti-Canine IgG Fc Secondary antibody tested against purified Canine IgG F(c). Each well was coated in duplicate with 1.0 ug of Canine IgG F(c) (red line), Canine IgG (green line) .The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Gt Anti-Rabbit HRP and substrate.

Applications for Rabbit anti-Canine IgG Fc Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Canine IgG Fc Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Canine IgG Fc Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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