Goat anti-Feline IgG Fc Secondary Antibody [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73464
Conjugate
Catalog #
Key Product Details
Species Reactivity
Feline
Applications
Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Cat IgG F(c) fragment
Specificity
No reaction was observed against Cat IgG F(ab).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Cat IgG, Cat IgG F(c) and Cat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Cat IgG, Cat IgG F(c) and Cat Serum
Scientific Data Images for Goat anti-Feline IgG Fc Secondary Antibody [FITC]
Dot Blot: Goat anti-Feline IgG Fc Secondary Antibody [FITC] [NBP1-73464] - Dot Blot of Goat anti-Feline IgG Fc Secondary antibody [FITC].Lane 1: 100 ng Cat F(c).Lanes 2-5: serial dilution 3 fold.Primary Antibody: none. Secondary Antibody: Goat Anti-Cat IgG F(c ) Antibody FITC at 1:1000 dilution at RT for 60 minutes. Block: 30 min at RT.
Applications for Goat anti-Feline IgG Fc Secondary Antibody [FITC]
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product has been tested by Dot Blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat anti-Feline IgG Fc Secondary Antibody [FITC]
Product Specific Notices for Goat anti-Feline IgG Fc Secondary Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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