Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73581
Conjugate
Catalog #
Key Product Details
Species Reactivity
Bovine
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Rabbit anti-Bovine IgG Fc fragment Secondary Antibody was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.
Specificity
No reaction was observed against Bovine IgG F(ab).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum
Scientific Data Images for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]
ELISA: Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP] [NBP1-73581] - ELISA Results of Rabbit anti-Bovine IgG Fc Secondary antibody [HRP] tested against purified Bovine IgG Fc HRP. Each well was coated in duplicate with 1.0 ug of Bovine IgG Fc. The working dilution is 1:15,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
Applications for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:2500
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
1:1000 - 1:10000
Application Notes
This product has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]
Product Specific Notices for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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