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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Purified Human IgG, Fc fragment

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, human IgA or IgM, and serum proteins from bovine, mouse, or rabbit

Specificity

Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against human IgA + IgM, bovine, horse, mouse, and rabbit serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.

Applications for Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)

Application
Recommended Usage

ELISA

1:200-1:5000

Immunohistochemistry

1:500-1:5000

Western Blot

1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Format

Pre-adsorbed

Preservative

0.1% Kathon

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Product Documents for Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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