Goat anti-Rabbit IgG Fc Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75346
Key Product Details
Species Reactivity
Rabbit
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Purified Rabbit IgG Fc
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins, light chains on all rabbit immunoglobulins, and Rabbit IgG F(ab)'2 fragment
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Applications for Goat anti-Rabbit IgG Fc Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:200-1:5000
Immunohistochemistry-Frozen
1:500-1:5000
Immunohistochemistry-Paraffin
1:500-1:5000
Western Blot
1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.1% Kathon
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat anti-Rabbit IgG Fc Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Rabbit IgG Fc Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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