Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75348
Key Product Details
Species Reactivity
Rabbit
Applications
Flow Cytometry, Immunomicroscopy
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Purified Rabbit IgG Fc
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins, light chains on all rabbit immunoglobulins, and Rabbit IgG F(ab)'2 fragment
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily
Applications for Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 488]
Application
Recommended Usage
Flow Cytometry
1:20-1:2000
Immunomicroscopy
1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 488]
Product Specific Notices for Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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