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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunomicroscopy

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Purified Human IgG, Fc fragment

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins

Specificity

Based on IEP, this antibody reacts with heavy gamma chains on human IgG

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Affinity purified antibody is >95% pure based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 550 (Ex = 550 nm; Em = 576 nm). After reconstitution, prepare fresh working dilution daily.

Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.

Scientific Data Images for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]

Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [DyLight 550] [NBP1-75816] - SKBR3 human breast cancer cell line. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [DyLight 550] [NBP1-75816] - MCF7 human breast cancer cell line. Image from verified customer review.

Applications for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]

Application
Recommended Usage

Flow Cytometry

1:20 - 1:2000

Immunomicroscopy

1:20 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-75816 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Product Documents for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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