Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75816
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunomicroscopy
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Purified Human IgG, Fc fragment
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Affinity purified antibody is >95% pure based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 550 (Ex = 550 nm; Em = 576 nm). After reconstitution, prepare fresh working dilution daily.
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Scientific Data Images for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]
Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [DyLight 550] [NBP1-75816] - SKBR3 human breast cancer cell line. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [DyLight 550] [NBP1-75816] - MCF7 human breast cancer cell line. Image from verified customer review.
Applications for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]
Application
Recommended Usage
Flow Cytometry
1:20 - 1:2000
Immunomicroscopy
1:20 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP1-75816 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: IgG Fc
UniProt
Additional IgG Fc Products
Product Documents for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]
Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [DyLight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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