Goat anti-Mouse IgG Fc Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60664
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Goat anti-Mouse IgG Fc fragment Secondary Antibody was produced by repeated immunization with Mouse IgG F(c) fragment in goat.
Specificity
No reaction was observed against Mouse IgG F(ab).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat anti-Mouse IgG Fc Secondary Antibody
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664]
Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image from the DyLight 488 version of this antibody.Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664]
Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Analysis of Vero cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. Image from verified customer review. Image using the DyLight 488 form of this antibody.Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664]
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 405 form of this antibody.Applications for Goat anti-Mouse IgG Fc Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:5000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:10000
Application Notes
This product has been tested by ELISA, dot blot, and wester blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat anti-Mouse IgG Fc Secondary Antibody
Product Specific Notices for Goat anti-Mouse IgG Fc Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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