Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Intracellular & surface Ig staining of P. alecto splenocytes. Dead cells were excluded using Live/Dead eFluor 506 dye & live cells were gated for singlets using forward scatter height (FSC-H) & area (FSC-A). For all the staining approaches (surface only, intracellular only, or surface + intracellular), a distinct sIg+ CD3+/− population is observed. Greater than 85% of this population is MHCII+. Comparable percentages of these cells were obtained with surface staining only vs. intracellular staining only, thus strongly suggesting that this population is truly a sIg+ B cell population. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30930908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.