Key Product Details

Species Reactivity

Goat

Applications

Flow Cytometry, Immunomicroscopy

Label

DyLight 800 (Excitation = 777 nm, Emission = 794 nm)

Antibody Source

Polyclonal Chicken IgG

Format

Pre-adsorbed

Concentration

LYOPH mg/ml

View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

This Chicken anti-Goat IgG (H+L) Secondary Antibody [DyLight 800] (Pre-adsorbed) was developed against purified goat IgG (H&L).

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins and IgG/serum proteins from human, mouse, or rabbit

Specificity

Based on IEP, this Chicken anti-Goat IgG (H+L) Secondary Antibody [DyLight 800] (Pre-adsorbed) heavy gamma chains on goat IgG and light chains on all goat immunoglobulins. This antibody has been pre-adsorbed against human, mouse or rabbit IgG and serum proteins.

Clonality

Polyclonal

Host

Chicken

Isotype

IgG

Description

Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.

Applications

Application
Recommended Usage

Flow Cytometry

1:20 - 1:2000

Immunomicroscopy

1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Format

Pre-adsorbed

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

Chicken products cannot be exported to Canada.

Affinity purified antibody is > 95% based on SDS-PAGE. Chicken Ig fraction was prepared using eggs from healthy hens of US origin. DyLight 800 (Ex = 777 nm; Em = 794 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.