Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73452
Conjugate
Catalog #
Key Product Details
Species Reactivity
Monkey
Applications
Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Rhodamine (Excitation = 570 nm, Emission = 590 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Goat anti-Monkey IgG (gamma chain) Secondary Antibody was produced by repeated immunization with monkey IgG gamma heavy chain in goat.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum
Scientific Data Images for Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine]
Western Blot: Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73452] - Detection of Monkey IgG Gamma Chain. 100ng of Monkey IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Anti-Monkey IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using the Bio-Rad VersaDoc 4000 MP. Molecular weight markers are in lane 1.
Western Blot: Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73452] - This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.
Applications for Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine]
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Heavy Chain
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Heavy Chain Products
Product Documents for Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine]
Product Specific Notices for Goat anti-Monkey IgG Heavy Chain Secondary Antibody [Rhodamine]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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