Key Product Details

Species Reactivity

Monkey

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Goat anti-Monkey IgG (gamma chain) Secondary Antibody was produced by repeated immunization with monkey IgG gamma heavy chain in goat.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Monkey IgG Heavy Chain Secondary Antibody

Western Blot: Goat anti-Monkey IgG Heavy Chain Secondary Antibody [NBP2-60692]

Western Blot: Goat anti-Monkey IgG Heavy Chain Secondary Antibody [NBP2-60692]

Western Blot: Goat anti-Monkey IgG Heavy Chain Secondary Antibody [NBP2-60692] - Detection of Monkey IgG Gamma Chain. 100ng of Monkey IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Anti-Monkey IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using the Bio-Rad VersaDoc 4000 MP. Molecular weight markers are in lane 1. Image using the Rhodamine form of this antibody.

Applications for Goat anti-Monkey IgG Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:75000 - 1:175000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG Heavy Chain

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Heavy Chain Products

Product Documents for Goat anti-Monkey IgG Heavy Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Monkey IgG Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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