Skip to main content

Rabbit anti-Guinea Pig IgG1 Secondary Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69515

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-69515

Key Product Details

Species Reactivity

Guinea Pig

Applications

Immunoelectrophoresis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Pools of purified normal IgG1 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Reactivity Notes

This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.

Specificity

The reactivity of the antiserum is directed to the Fc subunit of subclass IgG1. It does not react with other IgG2, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Clonality

Polyclonal

Host

Rabbit

Description

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Applications for Rabbit anti-Guinea Pig IgG1 Secondary Antibody

Application
Recommended Usage

Immunoelectrophoresis

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Precipitation assays.

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Reconstitution

Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Formulation

Whole antiserum

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Rabbit anti-Guinea Pig IgG1 Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Guinea Pig IgG1 Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...