Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG1 Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG1 heavy chain

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody

Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]

Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]

Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]

Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]

Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]

Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]

Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.
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Applications for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product is available in a variety of formats. Anti-Mouse IgG1 Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG1 Heavy Chain

Additional IgG1 Heavy Chain Products

Product Documents for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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