Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72793
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.bio-techne.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0005.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgG1 heavy chain
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.![Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.bio-techne.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72793-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.bio-techne.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0001.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.bio-techne.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72793-img0006.jpg "Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
![ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.bio-techne.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72793-img0012.jpg "ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Applications for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product is available in a variety of formats. Anti-Mouse IgG1 Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG1 Heavy Chain
Additional IgG1 Heavy Chain Products
Product Documents for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Product Specific Notices for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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