Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73885
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP] was produced by repeated immunization with mouse IgM whole molecule in rabbit.
Specificity
This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum
Scientific Data Images for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP]
Dot Blot: Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP] [NBP1-73885] - Dot Blot results of Rabbit anti-Mouse IgM (H+L) Secondary antibody [HRP].Dots are Mouse IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit anti-Mouse IgM -HRP at 1ug/mL for 1hr at RT.
Applications for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:10000
Application Notes
This secondary peroxidase conjugated antibody This product has been tested by dot blot and ELISA and is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Mouse IgM (H&L) peroxidase conjugated antibody has been assayed against 1.0 ug of mouse IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Gentamicin Sulfate
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgM (H+L)
Additional IgM (H+L) Products
Product Documents for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP]
Product Specific Notices for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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