Goat anti-Human Kappa Light Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB7463
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human Kappa Light Chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all human immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Goat anti-Human Kappa Light Chain Secondary Antibody
Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463]
Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image form verified customer review.Applications for Goat anti-Human Kappa Light Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:1000 - 1:30000
Immunocytochemistry/ Immunofluorescence
1:200- 1:2000
Immunohistochemistry
1:200- 1:2000
Immunohistochemistry-Paraffin
1:200- 1:2000
Western Blot
1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Reviewed Applications
Read 1 review rated 5 using NB7463 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Goat anti-Human Kappa Light Chain Secondary Antibody
Product Specific Notices for Goat anti-Human Kappa Light Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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