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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

1.5 mg/ml

Product Summary

Immunogen

Purified Human Kappa light chain

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, heavy chains on all human immunoglobulins, and lambda light chains on all human immunoglobulins

Specificity

Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins. This antibody has been pre-adsorbed against mouse serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Applications

Application
Recommended Usage

ELISA

1:2000 - 1:20000

Immunohistochemistry

1:500 - 1:5000

Western Blot

1:2000 - 1:20000
Application Notes
This conjugate is suitable for all immunoassay applications.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Format

Pre-adsorbed

Preservative

0.05% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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