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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunomicroscopy

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Purified Human Kappa light chain

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, heavy chains on all human immunoglobulins, and lambda light chains on all human immunoglobulins

Specificity

Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 650 (Ex = 652 nm; Em = 672 nm). After reconstituted, prepare fresh working dilution daily

Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.

Applications for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]

Application
Recommended Usage

Flow Cytometry

1:20 - 1:2000

Immunomicroscopy

1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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