Rabbit anti-Llama VHH Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95273
Key Product Details
Species Reactivity
Llama
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rabbit anti-Llama VHH Secondary Antibodywas prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein.
Specificity
Anti-VHH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Anti-Vhh antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Rabbit anti-Llama VHH Secondary Antibody
Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273]
Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273] - Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VHH. Primary antibody: Rabbit anti-Llama VHH Secondary Antibody at 1:1,000 at 4C. Secondary antibody: HRP Goat anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VHH.Rabbit anti-Llama VHH Secondary Antibody
Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VhH. Primary antibody: Rabbit anti-Rabbit anti-Llama VHH Secondary Antibodyat 1:1,000 o/n at 4C. Secondary antibody: HRP Gt-a-Rb IgGApplications for Rabbit anti-Llama VHH Secondary Antibody
Application
Recommended Usage
ELISA
1:10000-1:50000
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: VHH
Alternate Names
anti-VhH Antibody, camelid VhH Antibody, single-domain antibody (sdAb)
Additional VHH Products
Product Documents for Rabbit anti-Llama VHH Secondary Antibody
Product Specific Notices for Rabbit anti-Llama VHH Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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