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Rabbit anti-Llama VHH Secondary Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95273

Novus Biologicals, part of Bio-Techne
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NBP2-95273-100ul

Key Product Details

Species Reactivity

Llama

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Rabbit anti-Llama VHH Secondary Antibodywas prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein.

Specificity

Anti-VHH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Anti-Vhh antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Rabbit anti-Llama VHH Secondary Antibody

Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273]

Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273]

Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273] - Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VHH. Primary antibody: Rabbit anti-Llama VHH Secondary Antibody at 1:1,000 at 4C. Secondary antibody: HRP Goat anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VHH.
Rabbit anti-Llama VHH Secondary Antibody

Rabbit anti-Llama VHH Secondary Antibody

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VhH. Primary antibody: Rabbit anti-Rabbit anti-Llama VHH Secondary Antibodyat 1:1,000 o/n at 4C. Secondary antibody: HRP Gt-a-Rb IgG

Applications for Rabbit anti-Llama VHH Secondary Antibody

Application
Recommended Usage

ELISA

1:10000-1:50000

Western Blot

1:1000-1:5000
Application Notes
This product has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: VHH

Alternate Names

anti-VhH Antibody, camelid VhH Antibody, single-domain antibody (sdAb)

Additional VHH Products

Product Documents for Rabbit anti-Llama VHH Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Llama VHH Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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