Non-Human Primate CXCL10/IP-10 Cartridge

Name: Non-Human Primate CXCL10/IP-10 Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011473

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011473

Simple Plex Non-Human Primate IP-10 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.186 pg/mL
Assay Range:	0.6 - 920 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate CXCL10/IP-10 Cartridge Summary:	Simple Plex assay for the detection of non-human primate Interferon gamma Inducible Protein 10 (IP-10) in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate CXCL10/IP-10 Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate CXCL10/IP-10 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant IP-10.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	14	12
Mean (pg/mL)	5.65	283	5.49	297
Standard Deviation	0.347	14.3	0.212	11.7
CV%	6.1	5	3.9	3.9

Linearity

Samples containing and/or spiked with high concentrations of IP-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate CXCL10/IP-10 Cartridge

	Non-Human Primate CXCL10/IP-10 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Item	Value
Entrez Gene IDs	3627 (Human); 15945 (Mouse)
Alternate Names	C7, CRG-2, CRG2, CXCL10, IFI10, INP10, IP-10, SCYB10, chemokine (C-X-C motif) ligand 10, gIP-10, mob-1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate CXCL10/IP-10 Cartridge

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Protocols & Manuals

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