Non-Human Primate IFN-alpha Cartridge

Name: Non-Human Primate IFN-alpha Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011458

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011458

Simple Plex Non-Human Primate IFN-a assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.51 pg/mL
Assay Range:	1.46 - 13,870 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate IFN-alpha Cartridge Summary:	Simple Plex assay for the detection of non-human primate Interferon alpha (IFN-alpha ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Non-Human Primate IFN-alpha Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant IFN-alpha.
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=3)	115	111-119
EDTA Plasma (n=4)	93	85-101
Heparin Plasma (n=4)	93	85-101
Serum (n=5)	89	80-99

Linearity

Samples containing and/or spiked with high concentrations of IFN-alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate IFN-alpha Cartridge

	Non-Human Primate IFN-a Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha
Entrez Gene IDs	3440 (Human)
Alternate Names	IFN-alpha, IFN-alphaA, IFNA, IFNA2, IFNA2b, IFNalpha, INFA2, LeIF D, interferon alpha 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate IFN-alpha Cartridge

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Protocols & Manuals

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