Non-Human Primate IL-5 Cartridge

Name: Non-Human Primate IL-5 Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011303

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011303

Simple Plex Non-Human Primate IL-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.02 pg/mL
Assay Range:	0.13 - 1,248 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate IL-5 Cartridge Summary:	Simple Plex assay for the detection of non-human primate Interleukin 5 (IL-5) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate IL-5 Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate IL-5 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant IL-5.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	10	10
Mean (pg/mL)	22.5	999	19	886
Standard Deviation	1.69	54.9	1.9	73.3
CV%	7.5	5.5	10.1	8.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=6)	107	103-112
Heparin Plasma (n=6)	107	99-116
Serum (n=6)	106	100-112

Linearity

Samples containing and/or spiked with high concentrations of IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate IL-5 Cartridge

	Non-Human Primate IL-5 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Item	Value
Long Name	Interleukin 5
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names	B-cell differentiation factor I, BCDF mu, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL-5, IL-5T-cell replacing factor, IL5, TRF, TRFB cell differentiation factor I, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate IL-5 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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