Simple Plex Cytokine Screening Panel

Name: Simple Plex Cytokine Screening Panel
Brand: ProteinSimple
SKU: SPCKE-PS-010629

ProteinSimple, part of Bio-Techne | Catalog # SPCKE-PS-010629

COA

Simple Plex 32x8 panel containing IL-1b, IL-12 p70, IL-10 2nd Gen, IL-4 2nd Gen, IL-2, IFNg 3rd Gen, TNF-a 2nd Gen, and IL-6 3rd Gen with sample diluent SD13. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).

Key Product Details

Features:

The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-12 p70, IL-10 2nd gen, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 3rd gen in serum and plasma (EDTA/Heparin).

Sample Type & Volume Required Per Well	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity	IL-1 beta 0.4 pg/mL; IL-12 p70 0.46 pg/mL; IL-10 2nd gen 0.17pg/mL; IL-2 0.54 pg/mL; IFNg 3rd gen 0.17 pg/mL; TNF-a 0.3 pg/mL; IL-4 0.52 pg/mL; IL-6 3rd gen 0.11 pg/mL
Assay Range	IL-1 beta 0.4 - 1,530 pg/mL; IL-12 p70 0.46 - 2,570 pg/mL; IL-10 2nd gen 0.38 - 1,446 pg/mL; IL-2 0.54 - 2,050 pg/mL; IFNg 3rd gen 0.17 - 4,000 pg/mL; TNF-a 0.3 - 1,160 pg/mL; IL-4 0.52 - 2,020 pg/mL; IL-6 3rd gen 0.28 - 2,652 pg/mL

Technical Data
Citations

Kit Contents for Simple Plex Cytokine Screening Panel

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human IL-1b, IL-12 p70, IL-10 2nd gen, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 3rd gen.
Cross-reactivity	<0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
IL-1b/IL-1F2	n	16	16	16	16
	Mean (pg/mL)	8.66	446	8.42	410
	Standard Deviation	0.427	16.5	0.342	23.3
	CV (%)	4.9	3.7	4	5.7
IL-12 p70	n	16	16	16	16
	Mean (pg/mL)	36.2	1657	33.2	1587
	Standard Deviation	1.25	90	1.95	137
	CV (%)	3.4	5.4	5.9	8.6
IL-10 2nd gen	n	16	16	35	37
	Mean (pg/mL)	8.89	462	10.8	477
	Standard Deviation	0.193	16.0	0.940	24.5
	CV (%)	2.2	3.5	8.7	5.1
IL-2	n	16	16	16	16
	Mean (pg/mL)	15	648	13	608
	Standard Deviation	0.8	32.7	1.13	34
	CV (%)	5.3	5.1	8.7	5.6
IFN-g 3rd gen	n	16	16	16	16
	Mean (pg/mL)	21.1	1175	23.4	1192
	Standard Deviation	1.48	33.4	2.52	99.4
	CV (%)	7	2.8	10.8	8.3
TNF-a 2nd gen	n	16	16	16	16
	Mean (pg/mL)	6.99	383	0.662	36.9
	Standard Deviation	0.345	20.6	7.81	408
	CV (%)	4.9	5.4	8.5	9
IL-4 2nd gen	n	16	16	16	16
	Mean (pg/mL)	15.9	621	13.5	631
	Standard Deviation	1.2	60.8	1.55	77.1
	CV (%)	7.5	9.8	11.5	12.2
IL-6 3rd gen	n	16	16	40	40
	Mean (pg/mL)	23.1	876	23.6	1008
	Standard Deviation	0.899	72.1	1.82	77.7
	CV (%)	3.9	8.2	7.7	7.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
IL-1b	EDTA Plasma (n=4)	106	97-120
	Heparin Plasma (n=4)	99	99-112
	Serum (n=4)	105	96-112
IL-12 p70	EDTA Plasma (n=6)	87	73-117
IL-12 p70	Serum (n=6)	81	73-90
IL-10 2nd gen	EDTA Plasma (n=4)	91	84-93
	Heparin Plasma (n=4)	91	79-95
	CCM (n=2)	101	100-103
	Serum (n=4)	95	93-97
IL-2	EDTA Plasma (n=4)	86	69-100
	Heparin Plasma (n=4)	90	70-103
	Serum (n=4)	92	69-102
IFN-g 3rd gen	EDTA Plasma (n=4)	89	88-90
	Heparin Plasma (n=4)	90	83-99
	Cerebrospinal Fluid (n=4)	94	91-98
	Serum (n=4)	95	83-102
TNF-a 2nd gen	EDTA Plasma (n=4)	97	89-105
	Heparin Plasma (n=4)	89	77-96
	Cerebrospinal Fluid (n=6)	109	103-115
	Serum (n=4)	99	92-108
IL-4 2nd gen	EDTA Plasma (n=4)	88	83-92
	Heparin Plasma (n=4)	87	84-89
	Serum (n=4)	96	94-99
IL-6 3rd gen	EDTA Plasma (n=4)	92	86-102
	Heparin Plasma (n=4)	87	85-90
	Serum (n=4)	94	90-99
	CCM (n=2)	104	103-106
	Saliva (n=4)	102	102-102
	Urine (n=4)	98	93-104

Product Specifications for Simple Plex Cytokine Screening Panel

Species

Human

Preparation & Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Simple Plex Cytokine Screening Panel

ProteinSimple, part of Bio-Techne | Catalog # SPCKE-PS-010629

Key Product Details

Kit Contents for Simple Plex Cytokine Screening Panel

Product Specifications

Precision

Recovery

Product Specifications for Simple Plex Cytokine Screening Panel

Preparation & Storage

Product Documents for Simple Plex Cytokine Screening Panel

COA

Certificate of Analysis

CONTACT INFORMATION

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