Simple Plex Cytokine Storm Panel
ProteinSimple, part of Bio-Techne | Catalog # SPCKA-PS-009470
Simple Plex 16x4 panel containing IL-1 beta / IL-1F2, IL-6 3rd Gen, IL-8/CXCL8, and TNF-alpha 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).
Key Product Details
| Features: |
The Simple Plex Human Multianalyte Assay is for the detection of human IL-1 beta, IL-6 3rd gen, IL-8, and TNF-alpha in serum and plasma (EDTA/Heparin).
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Summary for Simple Plex Cytokine Storm Panel
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human IL-1 beta/IL-1F2, IL-6 3rd gen, IL-8/CXCL8, and TNF alpha. |
| Cross-reactivity | |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| IL-1 beta/IL-1F2 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 8.66 | 446 | 8.42 | 410 | |
| Standard Deviation | 0.427 | 16.5 | 0.342 | 23.3 | |
| CV (%) | 4.9 | 3.7 | 4 | 5.7 | |
| IL-6 3rd gen | n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 23.1 | 876 | 23.6 | 1008 | |
| Standard Deviation | 0.899 | 72.1 | 1.82 | 77.7 | |
| CV (%) | 3.9 | 8.2 | 7.7 | 7.7 | |
| IL-8/CXCL8 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 28.7 | 1362 | 27.3 | 1463 | |
| Standard Deviation | 1.41 | 89.8 | 1.41 | 125 | |
| CV (%) | 4.9 | 6.6 | 5.2 | 8.6 | |
| TNF alpha 2nd Gen | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 6.99 | 383 | 0.662 | 36.9 | |
| Standard Deviation | 0.345 | 20.6 | 7.81 | 408 | |
| CV (%) | 4.9 | 5.4 | 8.5 | 9 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| IL-1 beta/IL-1F2 | EDTA Plasma (n=4) | 106 | 97-120 |
| Heparin Plasma (n=4) | 99 | 99-112 | |
| Serum (n=4) | 105 | 96-112 | |
| IL-6 3rd gen | EDTA Plasma (n=4) | 92 | 86-102 |
| Heparin Plasma (n=4) | 87 | 85-90 | |
| Serum (n=4) | 94 | 90-99 | |
| CCM (n=2) | 104 | 103-106 | |
| Saliva (n=4) | 102 | 102-102 | |
| Urine (n=4) | 98 | 93-104 | |
| IL-8/CXCL8 | EDTA Plasma (n=5) | 89 | 75-102 |
| Heparin Plasma (n=5) | 95 | 88-102 | |
| Serum (n=3) | 92 | 88-97 | |
| TNF alpha 2nd Gen | EDTA Plasma (n=4) | 97 | 89-105 |
| Heparin Plasma (n=4) | 89 | 77-96 | |
| Serum (n=4) | 99 | 92-108 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-1 beta/IL-1F2, IL-6 2nd Gen, IL-8/CXCL8, and TNF alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the respective assays.
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