Simple Plex Discover Mouse/Rat Park7 Assay

Name: Simple Plex Discover Mouse/Rat Park7 Assay
Brand: ProteinSimple
SKU: SPCKB-MP-014257

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-014257

Simple Plex Mouse/Rat Park7 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	4.36 pg/mL
Assay Range:	32.8 - 20,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discover Mouse/Rat Park7 Assay Summary:	Simple Plex Discovery assay is for the detection of mouse and rat Parkinson Disease Gene 7 (Park7) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discover Mouse/Rat Park7 Assay

Technical Data
Citations

Product Specifications for Simple Plex Discover Mouse/Rat Park7 Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant Park7.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	33	33
Mean (pg/mL)	141	6963	153	7341
Standard Deviation	8.65	411	11.9	486
CV%	6.1	5.9	7.8	6.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	98	91-105

Linearity

Samples containing and/or spiked with high concentrations of Park7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Rat Park7 Ella Assay Linearity

Scientific Data Examples for Simple Plex Discover Mouse/Rat Park7 Assay

	Simple Plex Discovery Mouse/Rat Park7 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Park7/DJ-1

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.

Item	Value
Long Name	Parkinson Disease 7
Entrez Gene IDs	11315 (Human); 57320 (Mouse); 117287 (Rat)
Alternate Names	DJ-1, DJ1, DJ1FLJ34360, EC 3.4, FLJ27376, FLJ92274, Oncogene DJ1, Park7, Parkinson disease (autosomal recessive, early onset) 7, Parkinson disease protein 7, protein DJ-1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discover Mouse/Rat Park7 Assay

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Protocols & Manuals

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