Simple Plex Discovery Human Thrombomodulin Assay

Name: Simple Plex Discovery Human Thrombomodulin Assay
Brand: ProteinSimple
SKU: SPCKB-PS-012014

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012014

Simple Plex Human Thrombomodulin assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	1.99 pg/mL
Assay Range:	7.86 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human Thrombomodulin Assay Summary:	Simple Plex Discovery assay is for the detection of human Thrombomodulin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human Thrombomodulin Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human Thrombomodulin Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Thrombomodulin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	26	26
Mean (pg/mL)	89.1	4704	92.5	4626
Standard Deviation	7.15	251	7.94	377
CV%	8	5.3	8.6	8.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	98-104

Linearity

Samples containing and/or spiked with high concentrations of recombinant human Thrombomodulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human Thrombomodulin Assay

	Simple Plex Discovery Human Thrombomodulin Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Thrombomodulin/BDCA-3

Thrombomodulin (TM), also known as CD141 and BDCA3, is a transmembrane expressed on vascular endothelial cells (EC), arterial smooth muscle cells, monocytes, and macrophages. It binds Thrombin and enhances the Thrombin-mediated activation of the anti-coagulant Protein C and the anti-fibrinolytic TAFI/Carboxypeptidase B2. Thrombomodulin also inhibits the ability of Thrombin to activate several pro-coagulant proteins (e.g. Fibrinogen, Factor V, Factor XIII, PAR-1). Thrombomodulin exerts multiple anti-inflammatory effects and reduces the severity of disease involving chronic inflammation. In cancer, Thrombomodulin suppresses tumor angiogenesis, growth, and epithelial-mesenchymal transition. Soluble fragments of Thrombomodulin are elevated in the serum, urine, and synovial fluid during coagulation disorders, inflammation, and organ failure.

Item	Value
Entrez Gene IDs	7056 (Human); 21824 (Mouse); 83580 (Rat)
Alternate Names	BDCA-3, BDCA3, CD141, CD141 antigen, Fetomodulin, THBD, THRM, TM, Thrombomodulin

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human Thrombomodulin Assay

Product Datasheets

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Protocols & Manuals

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