Does the Simple Plex Endonuclease assay detect Denarase® and Benzonase®?

Yes, both Denarase® and Benzonase® are detectable in this assay.

What is the minimum required dilution (MRD) for bio-process samples?

All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.

Simple Plex Endonuclease Cartridge Best Seller

Name: Simple Plex Endonuclease Cartridge
Brand: ProteinSimple
SKU: SPCKC-OT-008612

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-OT-008612

Product Datasheet / COA

Simple Plex Endonuclease assay kit contains cartridge, sample diluent SD19, and wash buffer. Part # starting with ST01 ships in 2-3 days.

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bioprocess Samples (25 uL)
Sensitivity:	0.415 pg/mL
Assay Range:	1.97 - 18,750 pg/mL (Bioprocess Samples)
Simple Plex Endonuclease Cartridge Summary:	Simple Plex assay for the detection of Endonuclease in bioprocess samples. Denarase® and Benzonase® are detectable in this assay. Benzonase® is a registered trademark of Merck KGaA Corp. Denarase® is a registered trademark of c-LEcta GmbH.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Endonuclease Cartridge

Technical Data
Citations / FAQs

Product Specifications for Simple Plex Endonuclease Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Bioprocess
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	43
Mean (pg/mL)	191	5922	126	5636
Standard Deviation	12.2	194	14.6	576
CV%	6.4	3.3	11.6	10.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Bioprocess Samples (n=3)	94	90-97

Linearity

Samples containing and/or spiked with high concentrations Endonuclease were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Endonuclease Cartridge

	Endonuclease Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs for Simple Plex Endonuclease Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Endonuclease Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol