Simple Plex Human Adiponectin/Acrp30 Cartridge

Name: Simple Plex Human Adiponectin/Acrp30 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000337

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000337

Simple Plex Human Adiponectin/Acrp30 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	6.24 pg/mL
Assay Range:	33.8 - 51,540 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Adiponectin/Acrp30 Cartridge Summary:	Simple Plex assay for the detection of human Adiponectin in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Adiponectin/Acrp30 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Adiponectin/Acrp30 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Adiponectin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	37
Mean (pg/mL)	336	15446	304	14616
Standard Deviation	29.9	1004	24.1	985
CV%	8.9	6.5	7.9	6.7

Linearity

Samples containing and/or spiked with high concentrations of human Adiponectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Adiponectin/Acrp30 Cartridge

	Human Adiponectin/Acrp30 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Item	Value
Entrez Gene IDs	9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names	ACDC, ACRP30ADPN, ADIPQTL1, APM1APM-1, Acrp30, AdipoQ, Adiponectin, ApM1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein, adipocyte, C1Q and collagen domain containing, adiponectin, C1Q and collagen domain containing, apM-1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Adiponectin/Acrp30 Cartridge

Product Datasheets

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Protocols & Manuals

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