Simple Plex Human Angiogenin Cartridge

Name: Simple Plex Human Angiogenin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000471

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000471

Simple Plex Human Angiogenin/ANG assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.4 pg/mL
Assay Range:	0.49 - 4,680 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Angiogenin Cartridge Summary:	Simple Plex assay for the detection of human Angiogenin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Angiogenin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Angiogenin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Angiogenin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	10	10
Mean (pg/mL)	36.6	1616	34.4	1548
Standard Deviation	2.71	150	3.47	99
CV%	7.4	9.3	10.1	6.4

Linearity

Samples containing and/or spiked with high concentrations of human Angiogenin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Angiogenin Cartridge

	Human Angiogenin Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiogenin

Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.

Item	Value
Entrez Gene IDs	283 (Human)
Alternate Names	ALS9, ANG, Angiogenin, EC 3.1.27, EC 3.1.27.-, HEL168, MGC22466, MGC71966, RNASE5RNASE4, RNase 5, Ribonuclease 5, angiogenin, ribonuclease, RNase A family, 5, epididymis luminal protein 168

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Angiogenin Cartridge

Product Datasheets

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Protocols & Manuals

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