Simple Plex Human ASC Cartridge

Name: Simple Plex Human ASC Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000834

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000834

Product Datasheet / COA

Simple Plex Human ASC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	1.95 pg/mL
Assay Range:	5.24 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human ASC Cartridge Summary:	Simple Plex assay for the detection of human ASC in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human ASC Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human ASC Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human ASC.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	57	55
Mean (pg/mL)	65.2	3191	61.4	3062
Standard Deviation	3.21	170	6.23	294
CV%	4.9	5.3	10.1	9.6

Linearity

Samples containing and/or spiked with high concentrations of human ASC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human ASC Cartridge

	Human ASC Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ASC

ASC contains an N-terminal Pyrin domain and C-terminal CARD (Caspase-recruitment Domain), and is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli. Methylation of the ASC gene has been correlated with decreased protein expression in numerous types of cancer.

Item	Value
Long Name	Apoptosis-Associated Speck-Like Protein Containing A CARD
Entrez Gene IDs	29108 (Human)
Alternate Names	ASC, CARD5, Caspase recruitment domain-containing protein 5, PYCARD, PYD and CARD domain-containing protein, TMS, TMS-1, TMS1, Target of methylation-induced silencing 1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, hASC

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human ASC Cartridge

Product Datasheets

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COA

Protocols & Manuals

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