Simple Plex Human BDNF Cartridge

Name: Simple Plex Human BDNF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000389

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000389

Product Datasheet / COA

Simple Plex Human BDNF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL)
Sensitivity:	5.25 pg/mL
Assay Range:	9.44 - 36,000 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Simple Plex Human BDNF Cartridge Summary:	Simple Plex assay for the detection of human free BDNF in serum and platelet-poor plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human BDNF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human BDNF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human free BDNF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	20	22
Mean (pg/mL)	207	10079	219	11200
Standard Deviation	19.8	867	22.9	1144
CV%	9.6	8.6	10.5	10.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Platelet-poor EDTA Plasma (n=4)	82	76-86

Linearity

Samples containing and/or spiked with high concentrations of human free BDNF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human BDNF Cartridge

	Human free BDNF Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Item	Value
Long Name	Brain-derived Neurotrophic Factor
Entrez Gene IDs	627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names	ANON2, Abrineurin, BDNF, BULN2, MGC34632, Neurotrophin, brain-derived neurotrophic factor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human BDNF Cartridge

Product Datasheets

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COA

Protocols & Manuals

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