Simple Plex Human C-Reactive Protein/CRP Cartridge

Name: Simple Plex Human C-Reactive Protein/CRP Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000200

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000200

Simple Plex Human CRP assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	1.24 pg/mL
Assay Range:	32.8 - 50,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human C-Reactive Protein/CRP Cartridge Summary:	Simple Plex assay for the detection of human C-Reactive Protein (CRP) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human C-Reactive Protein/CRP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human C-Reactive Protein/CRP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CRP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	22
Mean (pg/mL)	291	18310	282	18318
Standard Deviation	19.6	783	14.2	1170
CV%	6.7	4.3	5	6.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=3)	99	97-101

Linearity

Samples containing and/or spiked with high concentrations of human CRP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human C-Reactive Protein/CRP Cartridge

	Human CRP Ella Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Item	Value
Entrez Gene IDs	1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names	C-Reactive Protein, C-reactive protein, pentraxin-related, CRP, MGC88244, PTX1MGC149895, pentraxin 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

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Product Documents for Simple Plex Human C-Reactive Protein/CRP Cartridge

Product Datasheets

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Protocols & Manuals

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