Simple Plex Human CA125/MUC16 Cartridge

Name: Simple Plex Human CA125/MUC16 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000475

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000475

Simple Plex Human CA125/MUC16 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	158 mU/mL
Assay Range:	309.28 - 471,920 mU/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CA125/MUC16 Cartridge Summary:	Simple Plex assay for the detection of human CA125 in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CA125/MUC16 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CA125/MUC16 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CA125.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	101	97-106
Heparin Plasma (n=4)	93	90-98
Serum (n=4)	95	88-99

Linearity

Samples containing and/or spiked with high concentrations of human CA125 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human CA125/MUC16 Cartridge

	Human CA125 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CA125/MUC16

The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.

Item	Value
Long Name	Ovarian Cancer Antigen CA125
Entrez Gene IDs	94025 (Human)
Alternate Names	CA-125, CA125, CA125 ovarian cancer antigen, CA125MUC-16, FLJ14303, MUC16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, mucin 16, cell surface associated, mucin-16

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CA125/MUC16 Cartridge

Product Datasheets

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Protocols & Manuals

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