Simple Plex Human CCL17/TARC Cartridge

Name: Simple Plex Human CCL17/TARC Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000194

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000194

Simple Plex Human CCL17/TARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.418 pg/mL
Assay Range:	0.56 - 850 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL17/TARC Cartridge Summary:	Simple Plex assay for the detection of human Thymus and Activationo-Regulated Chemokine (TARC) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL17/TARC Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL17/TARC Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TARC.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	14	13
Mean (pg/mL)	6.33	417	6.84	393
Standard Deviation	0.298	38.9	0.541	41.8
CV%	4.7	9.3	7.9	10.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	109	107-111
EDTA Plasma (n=4)	99	84-112
Heparin Plasma (n=4)	97	79-110
Serum (n=4)	115	98-122

Linearity

Samples containing and/or spiked with high concentrations of human TARC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CCL17/TARC Cartridge

	Human CCL17/TARC Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL17/TARC

CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.

Item	Value
Entrez Gene IDs	6361 (Human); 20295 (Mouse)
Alternate Names	ABCD-2, C-C motif chemokine 17, CC chemokine TARC, CCL17, MGC138273, SCYA17, SCYA17MGC138271, Small-inducible cytokine A17, T cell-directed CC chemokine, TARC, TARCA-152E5.3, Thymus and activation-regulated chemokine, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A (Cys-Cys), member 17

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL17/TARC Cartridge

Product Datasheets

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COA

Protocols & Manuals

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