Simple Plex Human CCL19/MIP-3 beta Cartridge

Name: Simple Plex Human CCL19/MIP-3 beta Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-002348

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002348

Simple Plex Human CCL19/MIP-3 beta assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	0.35 pg/mL
Assay Range:	1.57 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL19/MIP-3 beta Cartridge Summary:	Simple Plex assay for the detection of human MIP-3 beta in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL19/MIP-3 beta Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL19/MIP-3 beta Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MIP-3 beta.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	36
Mean (pg/mL)	36.4	2055	34.8	1881
Standard Deviation	1.57	115	3.51	209
CV%	4.3	5.6	10.1	11.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=3)	100	95-104
Heparin Plasma (n=3)	97	94-100
Serum (n=3)	101	100-103

Linearity

Samples containing and/or spiked with high concentrations of human MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CCL19/MIP-3 beta Cartridge

	Human MIP-3 beta Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Alternate Names

Beta-chemokine exodus-3, C-C motif chemokine 19, CC chemokine ligand 19, CCL19, CKb11, EBI1-ligand chemokine, ELC, ELCMIP-3-beta, Epstein-Barr virus-induced molecule 1 ligand chemokine, MGC34433, MIP-3 beta, MIP-3b, MIP3 beta, MIP3BCK beta-11, Macrophage inflammatory protein 3 beta, SCYA19EBI1 ligand chemokine, Small-inducible cytokine A19, beta chemokine exodus-3, chemokine (C-C motif) ligand 19, exodus-3, macrophage inflammatory protein 3-beta, small inducible cytokine subfamily A (Cys-Cys), member 19

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL19/MIP-3 beta Cartridge

Product Datasheets

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Protocols & Manuals

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