Simple Plex Human CCL20/MIP-3 alpha Cartridge

Name: Simple Plex Human CCL20/MIP-3 alpha Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001170

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001170

Simple Plex Human CCL20/MIP-3 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.084 pg/mL
Assay Range:	0.32 - 3,100 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL20/MIP-3 alpha Cartridge Summary:	Simple Plex assay for the detection of human CCL20/Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha ) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL20/MIP-3 alpha Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL20/MIP-3 alpha Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MIP-3 alpha.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	31	28
Mean (pg/mL)	21.1	992	19.9	918
Standard Deviation	1.3	55.3	1.9	67.6
CV%	6.2	5.6	9.7	7.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	103	100-106
EDTA Plasma (n=4)	102	97-107
Heparin Plasma (n=4)	100	92-111
Serum (n=4)	101	100-103

Linearity

Samples containing and/or spiked with high concentrations of human MIP-3 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CCL20/MIP-3 alpha Cartridge

	Human MIP-3 alpha Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.

Item	Value
Entrez Gene IDs	6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names	Beta-chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, LARCLiver and activation-regulated chemokine, MIP-3 alpha, MIP-3-alpha, MIP-3a, MIP3 alpha, MIP3AMacrophage inflammatory protein 3 alpha, SCYA20Small-inducible cytokine A20, ST38, beta chemokine exodus-1, chemokine (C-C motif) ligand 20, exodus-1, small inducible cytokine subfamily A (Cys-Cys), member 20

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL20/MIP-3 alpha Cartridge

Product Datasheets

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Protocols & Manuals

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