Simple Plex Human CCL4/MIP-1 beta Cartridge

Name: Simple Plex Human CCL4/MIP-1 beta Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000359

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000359

Simple Plex Human CCL4/MIP-1 beta assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	3.32 pg/mL
Assay Range:	12.1 - 2,950 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL4/MIP-1 beta Cartridge Summary:	Simple Plex assay for the detection of human MIP-1 beta in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL4/MIP-1 beta Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL4/MIP-1 beta Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MIP-1 beta.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	102	99
Mean (pg/mL)	16.6	863	16.7	915
Standard Deviation	0.632	32.9	1.04	37.2
CV%	3.8	3.8	6.3	4.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	111	106-115
EDTA Plasma (n=4)	103	91-109
Heparin Plasma (n=4)	98	90-105
Serum (n=4)	103	96-107

Linearity

Samples containing and/or spiked with high concentrations of human MIP-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human CCL4/MIP-1 beta Cartridge

	Human MIP-1 beta Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL4/MIP-1 beta

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

Entrez Gene IDs

6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)

Alternate Names

ACT-2, ACT2, AT744.1, C-C motif chemokine 4, CCL4, Exodus-3, G-26, G-26 T-lymphocyte-secreted protein, HC21, LAG-1, LAG1, Lymphocyte activation gene 1 protein, MIP-1 beta, MIP-1-beta, MIP-1-beta(1-69), MIP1 beta, MIP1B, MIP1B1, Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SCYA2, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, chemokine (C-C motif) ligand 4, secreted protein G-26, small inducible cytokine A4 (homologous to mouse Mip-1b)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL4/MIP-1 beta Cartridge

Product Datasheets

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Protocols & Manuals

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