Simple Plex Human CCL7/MCP-3 Cartridge

Name: Simple Plex Human CCL7/MCP-3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000477

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000477

Simple Plex Human CCL7/MCP-3/MARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.41 pg/mL
Assay Range:	0.41 - 1,550 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL7/MCP-3 Cartridge Summary:	Simple Plex assay for the detection of human Monocyte Chemotactic Protein 3 (MCP-3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL7/MCP-3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL7/MCP-3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MCP-3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	13	13
Mean (pg/mL)	9.31	492	9.18	47.4
Standard Deviation	0.482	30.6	0.625	43.4
CV%	5.2	6.2	6.8	9.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	116	111-121
EDTA Plasma (n=4)	99	93-105
Heparin Plasma (n=4)	90	85-96
Serum (n=4)	92	81-105

Linearity

Samples containing and/or spiked with high concentrations of human MCP-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CCL7/MCP-3 Cartridge

	Human CCL7/MCP-3 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL7/MCP-3/MARC

Human MCP-3 and mouse MARC are members of the CC subfamily of chemokines and are monocyte chemoattractants. Sequence comparisons suggest that MARC may be the mouse homolog of the human MCP-3 gene. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene.

Item	Value
Entrez Gene IDs	6354 (Human); 20306 (Mouse)
Alternate Names	C-C motif chemokine 7, CCL7, FIC, MARC, MCP-3, MCP-3Small-inducible cytokine A7, MCP3Monocyte chemotactic protein 3, MGC138465, Monocyte chemoattractant protein 3, NC28SCYA6, SCYA7MGC138463, chemokine (C-C motif) ligand 7, small inducible cytokine A7 (monocyte chemotactic protein 3)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL7/MCP-3 Cartridge

Product Datasheets

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Protocols & Manuals

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