Simple Plex Human CCL8/MCP-2 Cartridge

Name: Simple Plex Human CCL8/MCP-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000478

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000478

Simple Plex Human CCL8/MCP-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	0.05 pg/mL
Assay Range:	0.24 - 933 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL8/MCP-2 Cartridge Summary:	Simple Plex assay for the detection of human MCP-2 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL8/MCP-2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL8/MCP-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MCP-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	33	33
Mean (pg/mL)	5.15	201	5.3	227
Standard Deviation	0.266	16.6	0.598	16.5
CV%	5.2	8.3	11.3	7.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	97	93-102
Heparin Plasma (n=4)	97	86-103
Serum (n=4)	101	92-107

Linearity

Samples containing and/or spiked with high concentrations of human MCP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CCL8/MCP-2 Cartridge

	Human MCP-2 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL8/MCP-2

MCP-2 is a CC chemokine subfamily monocyte chemotactic protein. MCP-2 also chemoattracts activated NK cells, CD4+ and CD8+ T lymphocytes, and eosinophils and can induce histamine secretion from basophils. MCP-2 shares 62% amino acid sequence identity with MCP-1 and 58% with MCP-3.

Item	Value
Entrez Gene IDs	6355 (Human); 20307 (Mouse)
Alternate Names	C-C motif chemokine 8, CCL8, HC14, MCP-2, MCP2, SCYA10, chemokine (C-C motif) ligand 8, member 8 (monocyte chemotactic protein 2), monocyte chemoattractant protein 2, small-inducible cytokine A8

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL8/MCP-2 Cartridge

Product Datasheets

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Protocols & Manuals

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