Simple Plex Human CD14 Cartridge

Name: Simple Plex Human CD14 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000386

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000386

Product Datasheet / COA

Simple Plex Human CD14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL), Urine (500 nL)
Sensitivity:	1 pg/mL
Assay Range:	16.9 - 4,130 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human CD14 Cartridge Summary:	Simple Plex assay for the detection of human CD14 in serum, plasma (EDTA/Heparin), and urine

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CD14 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CD14 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CD14.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	31	31
Mean (pg/mL)	31.7	1308	28.1	1233
Standard Deviation	3.07	95.9	2.74	162
CV%	9.7	7.3	9.7	13.2

Linearity

Samples containing and/or spiked with high concentrations of human CD14 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CD14 Cartridge

	Human CD14 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD14

CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.

Item	Value
Entrez Gene IDs	929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names	CD14, CD14 antigen, CD14 molecule, Myeloid cell-specific leucine-rich glycoprotein, monocyte differentiation antigen CD14

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CD14 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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