Simple Plex Human CD163 Cartridge 2nd Generation

Name: Simple Plex Human CD163 Cartridge 2nd Generation
Brand: ProteinSimple
SKU: SPCKB-PS-007509

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-007509

Simple Plex Human CD163 2nd Gen assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	3.23 pg/mL
Assay Range:	19.8 - 12,088 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CD163 Cartridge 2nd Generation Summary:	Simple Plex assay for the detection of human CD163 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CD163 Cartridge 2nd Generation

Technical Data
Citations

Product Specifications for Simple Plex Human CD163 Cartridge 2nd Generation

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CD163.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	28	30
Mean (pg/mL)	191	9427	87	4299
Standard Deviation	8.3	641	10.2	400
CV%	4.3	6.8	11.7	9.3

Linearity

Samples containing and/or spiked with high concentrations of human CD163 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human CD163 Cartridge 2nd Generation

	Human CD163 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Item	Value
Entrez Gene IDs	9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names	CD163, CD163 molecule, CD_antigen: CD163, GHI/61, HbSR, Hemoglobin scavenger receptor, M130, MM130, RM3/1, SCARI1, Soluble CD163, macrophage-associated antigen, sCD163, scavenger receptor cysteine-rich type 1 protein M130

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human CD163 Cartridge 2nd Generation

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol